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αil-5  (Mabtech Inc)

 
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    Mabtech Inc αil-5
    αil 5, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/αil-5/product/Mabtech Inc
    Average 90 stars, based on 1 article reviews
    αil-5 - by Bioz Stars, 2026-06
    90/100 stars

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    (A) H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (B) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of intestinal granulomas. (C) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed by flow cytometry for quantitation of SILP myeloid cells. (D) Wild type mice were rechallenged with H. polygyrus concurrent with control IgG, αGM-CSF, αIL-5, or αGM-CSF plus αIL-5 antibody treatment and were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (E) Naïve (open circles) and H. polygyrus-rechallenged mice (filled triangles) treated as in (D) were analyzed for quantitation of intestinal granulomas. Data are pooled from 2 independent experiments (A-C) or are from 1 experiment (D and E). Data are mean ± s.e.m. Significance calculated with an unpaired Student’s t-test.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: BHLHE40 promotes T H 2 cell-mediated anti-helminth immunity and reveals cooperative CSF2RB family cytokines

    doi: 10.4049/jimmunol.1900978

    Figure Lengend Snippet: (A) H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (B) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of intestinal granulomas. (C) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed by flow cytometry for quantitation of SILP myeloid cells. (D) Wild type mice were rechallenged with H. polygyrus concurrent with control IgG, αGM-CSF, αIL-5, or αGM-CSF plus αIL-5 antibody treatment and were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (E) Naïve (open circles) and H. polygyrus-rechallenged mice (filled triangles) treated as in (D) were analyzed for quantitation of intestinal granulomas. Data are pooled from 2 independent experiments (A-C) or are from 1 experiment (D and E). Data are mean ± s.e.m. Significance calculated with an unpaired Student’s t-test.

    Article Snippet: The following antibodies were purchased from Becton Dickinson: αCD45 BV510 (30-F11), αGM-CSF BV421 (MP1–22E9), and αSiglec-F PE (E50–2440). αIL-5 FITC (TRFK5) was purchased from Leinco.

    Techniques: Quantitation Assay, Flow Cytometry

    (A) H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (B) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of intestinal granulomas. (C) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed by flow cytometry for quantitation of SILP myeloid cells. (D) Wild type mice were rechallenged with H. polygyrus concurrent with control IgG, αGM-CSF, αIL-5, or αGM-CSF plus αIL-5 antibody treatment and were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (E) Naïve (open circles) and H. polygyrus-rechallenged mice (filled triangles) treated as in (D) were analyzed for quantitation of intestinal granulomas. Data are pooled from 2 independent experiments (A-C) or are from 1 experiment (D and E). Data are mean ± s.e.m. Significance calculated with an unpaired Student’s t-test.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: BHLHE40 promotes T H 2 cell-mediated anti-helminth immunity and reveals cooperative CSF2RB family cytokines

    doi: 10.4049/jimmunol.1900978

    Figure Lengend Snippet: (A) H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (B) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed for quantitation of intestinal granulomas. (C) Naïve and H. polygyrus-rechallenged Csf2rb+/+ and Csf2rb−/− mice were analyzed by flow cytometry for quantitation of SILP myeloid cells. (D) Wild type mice were rechallenged with H. polygyrus concurrent with control IgG, αGM-CSF, αIL-5, or αGM-CSF plus αIL-5 antibody treatment and were analyzed for quantitation of H. polygyrus eggs/gram feces over time. (E) Naïve (open circles) and H. polygyrus-rechallenged mice (filled triangles) treated as in (D) were analyzed for quantitation of intestinal granulomas. Data are pooled from 2 independent experiments (A-C) or are from 1 experiment (D and E). Data are mean ± s.e.m. Significance calculated with an unpaired Student’s t-test.

    Article Snippet: For in vivo antibody blockade, mice were injected i.p. with 300 μg of αGM-CSF (Leinco, MP1–22E9), αIL-5 (BioXCell, TRFK5), or control polyclonal rat IgG (Sigma Aldrich I4131) every other day from the day of reinfection.

    Techniques: Quantitation Assay, Flow Cytometry